Vidic instructs Desmond to relive the early years of Altaïr Ibn-La'Ahad ( Philip Shahbaz), a senior member of the Assassin Brotherhood during the Third Crusade.

To perform many assassinations and other tasks, the player must consider the use of actions distinguished by their type of profile. Assassin's Creed II was released in the United States and Canada on November 17, 2009, and in Europe on November 20, 2009. In Assassin's Creed Odyssey, Layla recovers the Spear of Leonidas, from which she extracts the DNA of Leonidas' grandchildren, Alexios and Kassandra.Gameplay revolves around combat, stealth, and exploration, including the use of parkour to navigate the environment. The 12th-century portion of the story follows Altaïr, an Assassin, who embarks on a quest to regain his honour after botching a mission to recover one such artifact from the Templars; he does so by finding and assassinating nine targets across the Holy Land. To find the latter, Desmond uses the Animus to relive the memories of two of his ancestors who were in possession of the key at various points in time: Haytham Kenway, a British Templar from the 18th century who travels to the American colonies to establish a strong Templar presence on the continent and find the Grand Temple; and Ratonhnhaké:ton (later known as Connor), Haytham's half- Mohawk son, who becomes an Assassin to exact revenge on the Templars, whom he blames for the destruction of his village and the death of his mother in his youth.

s mixed review for Electronic Gaming Monthly, he wrote that the game features "a challenging parkour path of escape", and while intriguing, it is "an incomplete template based on multiple other games". The side quest prerequisite was one of the most criticized aspects of the game, so it was abandoned in future games.

Brotherhood shares many of the same features as the previous game though it takes place primarily in one city: Rome. Layla travels to the temple, using the Staff to protect herself from the radiation inside, and enters the Grey, a virtual world created by the Isu, where she meets both the Reader (implied to be Desmond's preserved consciousness) and Basim Ibn Ishaq, a Hidden One and Sage (the reincarnation of the Isu Loki) who was trapped in the Grey by Eivor. With the help of Malik and several Assassins brought for backup, Altaïr storms the citadel and confronts Al Mualim in the gardens, resisting the Apple's powers and killing his mentor.

John is later revealed to be a Sage, a human reincarnation of Juno's husband Aita, who is trying to resurrect her, though he is killed by Abstergo before his plan can come to fruition. Among this work was the idea of the Animus, which came about after the team decided to focus on the Assassins.These additions became a central part of the series going forward as well as other Ubisoft games like Watch Dogs, Far Cry, and Tom Clancy's Ghost Recon.

In May 2014, Guillemot stated that, "for the foreseeable future", Assassin's Creed games would continue releasing on the last generation PlayStation 3 and Xbox 360, despite the franchise moving to the current generation PlayStation 4 and Xbox One with Unity. There, the agent, in addition to providing a safe house, gives the player minimal knowledge about the target and requires them to perform additional reconnaissance missions before attempting the assassination. According to the interview, the plot centers on genetic memory and a corporation looking for descendants of an assassin. The player may also take part in several side objectives, including climbing tall towers to map out the city and saving citizens being threatened or harassed by the city guards. As Microsoft and Sony had not yet revealed their next consoles, the game's initial development from January 2004 was aimed as a PlayStation 2 title, following the same linear approach that The Sands of Time had taken.Main games in the Assassin's Creed series are set in an open world and played from the third-person view.